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abcam ab72527Anti-CTHRC1 antibody

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  • 上海力敏實(shí)業(yè)有限公司
  • 2015-11-28 00:48:42
  • 上海市
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  • 13634112423QQ:2856177234

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【簡(jiǎn)單介紹】

Tested applications
IHC-P, IHC-Fr, WB, ICC/IF
Cross reactivity
Reacts with
Mouse, Rat, Human, Pig

【詳細(xì)說(shuō)明】

Overview

Product name

Anti-CTHRC1 antibody [16D04]
See all CTHRC1 products （5） ...

Description

Mouse monoclonal [16D04] to CTHRC1

Tested applications

IHC-P, IHC-Fr, WB, ICC/IFmore details

Cross reactivity

 

Reacts with

Mouse, Rat, Human, Pig

 

Immunogen

Bacterial expressed full-length rat CTHRC1, reduced and non-reduced

Epitope

ab72527 recognises an epitope located at N Terminal region of CTHRC1 between Serine 32 and Valine 49

General notes

P.I. 7.5-7.8

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 150mM PBS

Concentration

 

  • 200 µg at 1.5mg/ml

Purity

Protein A purified

Purification notes

Purified from ascites.

Clonality

Monoclonal

Clone number

16D04

Isotype

IgG2b

Light chain type

kappa

Research areas

Cardiovascular >> Vasculature >> Vasculature Markers >> Arterial
Cancer >> Tumor biomarkers >> Other
Cancer >> Tumor immunology >> Tumor-associated antigens
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Signal Transduction >> Growth Factors/Hormones >> TGF
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
 

  • Datasheet PDF

  • Western blot - CTHRC1 antibody [16D04] （ab72527）Western blot - CTHRC1 antibody [16D04] （ab72527） image（enlarge）

  • Immunocytochemistry/ Immunofluorescence - CTHRC1 antibody [16D04] （ab72527）Immunocytochemistry/ Immunofluorescence - CTHRC1 antibody [16D04] （ab72527） image（enlarge）

  • Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - Anti-CTHRC1 antibody [16D04] （ab72527）Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - [16D04] （ab72527） image（enlarge）

Applications

Show applications key

Our Abpromise guarantee covers the use of ab72527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    IHC-P

    IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • ShowHide1 Image

    IHC-Fr

    （1 Abreview）IHC-Fr: Use at an assay ...Read more →

    IHC-Fr: Use at an assay dependent concentration. PubMed: 21596919

  • ShowHide1 Image

    WB

    WB: 1/10000 - 1/40000. P...Read more →

    WB: 1/10000 - 1/40000. Predicted molecular weight: 27 kDa.Unfortunay, recent testings indicate that ab72527 does not bind to recombinant human Cthrc1 in Western blot.

  • 1 Image

    ICC/IF

    ICC/IF: 1/1000.

    ICC/IF: 1/1000.

Target

Function

May act as a negative regulator of collagen matrix deposition.

Tissue specificity

Isoform 1 is expressed in calcified atherosclerotic plaque and chondrocyte-like cells.

Sequence similarities

Contains 1 collagen-like domain.

Post-translational
modifications

N-glycosylated.

Cellular localization

Secreted > extracellular space > extracellular matrix.

Information by UniProt

Alternative names

  • Collagen triple helix repeat containing 1 antibody
  • Collagen triple helix repeat containing protein 1 antibody
  • Collagen triple helix repeat-containing protein 1 antibody
see all

Database links

  • Entrez Gene: 115908 Human
  • Entrez Gene: 68588 Mouse
  • Entrez Gene: 282836 Rat
  • Omim: 610635 Human
  • SwissProt: Q96CG8 Human
  • SwissProt: Q9D1D6 Mouse
  • SwissProt: Q8CG08 Rat
  • Unigene: 405614 Human
see all

[16D04] images:

Western blot - CTHRC1 antibody [16D04] （ab72527）

Western blot - CTHRC1 antibody [16D04] （ab72527）

 

 

Lane 1 : [16D04] （ab72527） at 1/10000 dilution
Lane 2 : [16D04] （ab72527） at 1/20000 dilution
Lane 3 : [16D04] （ab72527） at 1/40000 dilution

Lane 1 : non reduced CHo full lenght CTHRC1 conditioned media
Lane 2 : non reduced CHo full lenght CTHRC1 conditioned media
Lane 3 : non reduced CHo full lenght CTHRC1 conditioned media


Predicted band size : 27 kDa
Observed band size : 55,70 kDa （why is the actual band size different from the predicted?）
 

 

Immunocytochemistry/ Immunofluorescence - CTHRC1 antibody [16D04] （ab72527）

Immunocytochemistry/ Immunofluorescence - CTHRC1 antibody [16D04] （ab72527）

 

 

CHO cells transduced with a full-length CTHRC1 virus. CTHRC1 is shown in red and nuclei in Blue. ab72527 dilution is 1:1000

 

Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - [16D04] （ab72527）

Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - [16D04] （ab72527）

 

 

IHC image of ab72527 staining in human melanoma formalinfixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA （pH9, epitope retrieval solution 2） for 20 mins. The section was then incubated with ab72527, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems （automated and non-automated） customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
 

    
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